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Updated: Feb 6, 2026

Plaquing of Herpes Simplex Viruses
Published on: November 5, 2021
Herpes simplex virus type 2: a cause of acute retinal necrosis syndrome
N N Markomichelakis1, P Zafirakis, P Karambogia-Karefillidi
1Department of Ophthalmology, General Hospital of Athens, Athens Medical School, Athens, Greece. marnik@otenet.gr
Purpose:
To describe a case of herpes simplex virus type 2 (HSV-2) acute retinal necrosis syndrome (ARN) in a 13-year-old immunocompetent girl.
Methods:
Polymerase chain reaction (PCR), cultures, flow cytometry, and cytology were performed on the vitreous sample.
Results:
Both PCR studies and vitreous cultures revealed HSV-2 as the cause of ARN. Flow cytometry showed CD4+, CD8+, and natural killer cells. The visual outcome of the patient was 20/200.
Conclusion:
Successful culture of HSV-2 from the vitreous specimen in a patient with ARN proved HSV-2 to be one of the causes of ARN. The successful culture of HSV-2 has not been previously reported.
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Necrosis
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...

