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[Changes in the rat cerebral cortex ultrastructure following peroral administration of manganese chloride]
T Z Bikashvili1, A A Shukakidze, G I Kiknadze
1Laboratory of Ultrastructural Study of Brain, Institute of Physiology Academy of Sciences of Georgia, Tbilisi.
Abstract:
The effect of 30 days treatment with MnCl(2).4H2O (50 mg/kg in doze) on neuron ultrastructure and interneuronal contacts of cerebral cortex frontoparietal area was studied in rat. The material for the research was taken on 1st (group 1) and 4th (group 2) month of the experiment. Changes in neurons and cortex synapses were less significant than those in subcortical structures. In 1st group of experimental animals neurons displayed vacuolar system hyperplasia and swelling of a part of mitochondria. Changes in synapses were heterogenous from reduction of synaptic vesicles number and mitochondria swelling to degeneration after "dark" type. Despite the fact that intoxication with MnCl(2).4H2O was over, changes in cortical neurons in group 2 were significant although no cells with signs of reorganization were observed. In neuropil of rats from group 2 reduced number of synaptic vesicles as well as reorganized endings filled with amorphous osmiophilic content were encountered more often as compared to animals from group 2.
Insights
Manganese chloride (MnCl2.4H2O) exposure alters rat brain neuron and synapse structure. While some changes persisted after treatment, no cellular reorganization was observed, indicating potential long-term effects.
Area of Science:
- Neuroscience
- Toxicology
- Cell Biology
Context:
- Investigating the impact of heavy metal exposure on neural tissues.
- Understanding the long-term consequences of manganese chloride (MnCl2.4H2O) intoxication on the central nervous system.
Purpose:
- To examine the ultrastructural changes in rat cerebral cortex neurons and synapses following MnCl2.4H2O treatment.
- To assess the persistence and nature of these changes at different time points post-exposure.
Summary:
- Rats treated with MnCl2.4H2O (50 mg/kg) for 30 days showed alterations in cortical neuron vacuolar systems and mitochondria.
- Synaptic changes included reduced vesicle numbers and degeneration. Even after intoxication ceased, significant neuronal alterations persisted, with increased evidence of synaptic vesicle depletion and terminal reorganization in the later experimental group.
Impact:
- This study highlights the persistent neurotoxic effects of manganese chloride on neuronal and synaptic structures.
- Findings suggest potential for long-term neurological deficits following manganese exposure, impacting interneuronal communication.