Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microarray-based analysis of early development in Xenopus laevis.

C R Altmann1, E Bell, A Sczyrba

  • 1Laboratory of Molecular Vertebrate Embryology, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.

Developmental Biology
|July 18, 2001
PubMed
Summary

Researchers developed Xenopus laevis cDNA microarrays for global transcriptional regulation studies in early vertebrate development. This approach identified novel temporally and spatially regulated genes, advancing molecular embryology research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The representation of individuals from ethnically minoritised groups in pain science education randomised controlled trials: a scoping review.

Musculoskeletal science & practice·2026
Same author

The AADOCR Position Statement on Community Water Fluoridation.

Journal of dental research·2025
Same author

Healthcare professionals' experiences of delivering pain science education to adults from ethnically minoritised groups.

Musculoskeletal science & practice·2024
Same author

Accuracy of heart rate measured by military-grade wearable ECG monitor compared with reference and commercial monitors.

BMJ military health·2023
Same author

Organotypic human lung bud microarrays identify BMP-dependent SARS-CoV-2 infection in lung cells.

Stem cell reports·2023
Same author

"Much better than I thought it was going to be": Telehealth delivered group-based education and exercise was perceived as acceptable among people with knee osteoarthritis.

Osteoarthritis and cartilage open·2022

Area of Science:

  • Developmental Biology
  • Genomics
  • Molecular Embryology

Background:

  • Understanding transcriptional regulation is crucial for deciphering early vertebrate embryonic development.
  • Existing methods may not offer a global view of gene expression patterns.

Purpose of the Study:

  • To develop and validate Xenopus laevis cDNA microarrays for global transcriptional analysis during early development.
  • To identify novel genes regulated temporally and spatially, and in response to signaling pathways.

Main Methods:

  • Preparation of prototype Xenopus laevis gastrula cDNA microarrays (864 sequences).
  • Development of a microarray analysis pipeline integrated with sequence analysis and annotation tools.
  • Experimental validation using RT-PCR and in situ hybridization.

Related Experiment Videos

Main Results:

  • Identification of temporally regulated genes by comparing maternal and zygotic transcription.
  • Discovery of novel genes with spatial enrichment in dorsal versus ventral mesoderm.
  • Characterization of novel genes regulated by activin signaling in embryonic explants.

Conclusions:

  • Xenopus laevis cDNA microarrays provide a powerful tool for global transcriptional profiling in molecular embryology.
  • Optimized protocols enable accurate and reproducible assessment of gene expression dynamics.
  • This approach facilitates the discovery of novel genes involved in embryonic development.