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Related Experiment Videos

Aptamer-based folding fluorescent sensor for cocaine.

M N Stojanovic1, P de Prada, D W Landry

  • 1Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, 630 West and 168th Street, New York, New York 10032, USA.

Journal of the American Chemical Society
|July 18, 2001
PubMed
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This study developed a novel deoxyribonucleotide aptamer sensor for cocaine detection. The aptasensor undergoes a structural change upon cocaine binding, enabling sensitive and selective signal generation for drug screening.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Aptamers are nucleic acid-based molecules that can bind to specific targets.
  • Developing sensitive and selective sensors for illicit substances like cocaine is crucial for detection and screening.
  • Existing methods for cocaine detection may lack sensitivity, selectivity, or require complex procedures.

Purpose of the Study:

  • To engineer a deoxyribonucleotide aptamer capable of signaling cocaine recognition.
  • To develop a novel sensor for the detection and quantification of cocaine.
  • To assess the sensor's performance in complex biological matrices and its utility for enzyme screening.

Main Methods:

  • Adaptation of a deoxyribonucleotide aptamer through a two-step process.

Related Experiment Videos

  • Engineering instability in a three-way junction stem to create a cocaine-binding pocket.
  • End-labeling the modified stem with a fluorophore and quencher for signal generation.
  • Monitoring structural changes (stem opening/closing) in response to cocaine binding.

    • Main Results:

    • The engineered aptamer sensor demonstrated a significant structural change upon cocaine binding.
    • The sensor exhibited fluorescence quenching in the absence of cocaine and signal generation in its presence.
    • The sensor showed selectivity for cocaine over its metabolites.
    • The aptasensor was functional and effective in serum samples.
    • The sensor proved useful for screening cocaine hydrolases.

    Conclusions:

    • A novel, structurally dynamic aptamer sensor for cocaine detection has been successfully developed.
    • The sensor provides a sensitive and selective method for cocaine identification and quantification.
    • The aptasensor's ability to function in serum and aid in enzyme screening highlights its practical applications.