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In vivo dynamics of human stem cell repopulation in NOD/SCID mice
J E Dick1, G Guenechea, O I Gan
1Programs in Cancer/Blood Research and Gene Therapy, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada, M5G 1X8. dick@sickkids.on.ca
Annals of the New York Academy of Sciences
|July 19, 2001
Abstract:
Primitive human hematopoietic cells can be assayed on the basis of their ability to repopulate immune-deficient NOD/SCID mice and have been termed SCID repopulating cells (SRCs). The in vivo biological fate of individual SRCs can be tracked by following the unique retroviral insertion site in the progency of transduced SRCs. Distinct human SRCs were identified that differ in the proliferative and self-renewal capacity indicating that the primitive cell compartment is functionally heterogeneous.