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Related Experiment Videos

Stem cell plasticity in muscle and bone marrow.

M A Goodell1, K A Jackson, S M Majka

  • 1Center for Cell and Gene Therapy, Baylor College of Medicine, One Baylor Plaza, N1030, Houston, Texas 77030, USA. goodell@bcm.tmc.edu

Annals of the New York Academy of Sciences
|July 19, 2001
PubMed
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Skeletal muscle stem cells can generate all major blood lineages, offering new therapeutic potential. Bone marrow stem cells also show promise in repairing cardiac muscle and blood vessel formation after injury.

Area of Science:

  • Stem cell biology
  • Hematopoiesis
  • Regenerative medicine

Background:

  • Tissue-derived stem cells exhibit remarkable plasticity.
  • Potential reciprocal relationship between skeletal muscle and bone marrow stem cells is unexplored.
  • Cell plasticity raises questions about lineage relationships and therapeutic applications.

Purpose of the Study:

  • To investigate the hematopoietic differentiation capacity of skeletal muscle-derived stem cells.
  • To examine the potential of bone marrow stem cells in cardiac repair and neovascularization.
  • To explore the reciprocal plasticity between skeletal muscle and bone marrow stem cells.

Main Methods:

  • Enzymatic digestion and in vitro culture of adult mouse skeletal muscle cells.
  • Transplantation of cultured muscle cells into lethally irradiated recipients with whole bone marrow.

Related Experiment Videos

  • Transplantation of purified bone marrow stem cells into mice with experimentally induced ischemic injury.

    • Main Results:

    • Cultured muscle-derived cells demonstrated robust hematopoietic differentiation, contributing 56% to peripheral blood lineages.
    • Muscle-derived cells showed 10- to 14-fold greater hematopoietic activity than whole bone marrow.
    • Bone marrow stem cells differentiated into cardiomyocytes and endothelial cells, contributing to cardiac repair and neovascularization.

    Conclusions:

    • Adult skeletal muscle contains stem cells with significant hematopoietic differentiation potential.
    • Bone marrow stem cells can differentiate into cardiac and endothelial cells, aiding tissue repair.
    • Demonstrated reciprocal plasticity suggests novel therapeutic avenues for regenerative medicine.