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Related Experiment Videos

Assembling filamentous phage occlude pIV channels.

Denise K Marciano1, M Russel, S M Simon

  • 1The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.

Proceedings of the National Academy of Sciences of the United States of America
|July 19, 2001
PubMed
Summary
This summary is machine-generated.

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Filamentous phage f1 exit E. coli via protein pIV channels. Exiting phages block oligosaccharide transport, indicating phages occupy these large aqueous channels during viral export.

Area of Science:

  • Microbiology
  • Structural Biology
  • Virology

Background:

  • Filamentous phage f1 are exported from Escherichia coli without lysis.
  • Phage protein pIV forms large channels in the E. coli outer membrane, proposed for phage extrusion.
  • The mechanism of phage f1 export through pIV channels remains unelucidated.

Purpose of the Study:

  • To investigate the role of pIV channels in filamentous phage f1 export.
  • To provide in vivo evidence for phage extrusion through pIV channels.

Main Methods:

  • Utilized a mutant pIV protein enabling channel opening independent of phage extrusion.
  • Developed an in vivo assay in E. coli lacking LamB.
  • Assessed oligosaccharide transport across the outer membrane in the presence of phage production.

Related Experiment Videos

Main Results:

  • The mutant pIV facilitated oligosaccharide transport in E. coli lacking LamB.
  • Oligosaccharide entry was reduced by phage production.
  • Oligosaccharide transport was further decreased by production of non-releasable phage.

Conclusions:

  • Exiting phage f1 physically block pIV channels.
  • This suggests phage occupy the lumen of pIV channels during export.
  • Provides the first evidence for viral exit through large aqueous channels.