Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evaluation of peptide electropherograms by multivariate mathematical-statistical methods. I. Principal component

I Miksík1, A Eckhardt, T Cserháti

  • 1Institute of Physiology, Academy of Sciences, Prague, Czech Republic. miksik@biomed.cas.cz

Journal of Chromatography. A
|July 20, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathogenic Role of mTOR Signaling in Cardiometabolic Disease: Implications for Heart, Liver, and Kidney Dysfunction.

Physiological research·2025
Same author

Altered Balance between Vasoconstrictor and Vasodilator Systems in Experimental Hypertension.

Physiological research·2025
Same author

Mechanisms Controlling the Behavior of Vascular Smooth Muscle Cells in Hypoxic Pulmonary Hypertension.

Physiological research·2024
Same author

Research on Experimental Hypertension in Prague (1966-2009).

Physiological research·2024
Same author

Gliflozins in the Treatment of Non-diabetic Experimental Cardiovascular Diseases.

Physiological research·2024
Same author

Corrigendum for: Blood Pressure Reduction Induced by Chronic Intracerebroventricular or Peroral Clonidine Administration in Rats With Salt-Dependent or Angiotensin II-Dependent Hypertension.

Physiological research·2023

Assessing protein glycation and fatty acid metabolite damage is challenging due to low concentrations. This study uses deep enzymatic fragmentation and capillary electrophoresis to reveal these post-translational modifications in collagen, aiding in understanding physiological condition impacts.

Area of Science:

  • Biochemistry
  • Proteomics
  • Analytical Chemistry

Background:

  • Assessing low-concentration protein modifications like glycation and fatty acid metabolite products is analytically challenging.
  • These modifications, arising from physiological conditions, can alter protein structure and function.
  • Collagen serves as a model protein to study non-enzymatic post-translational modifications.

Purpose of the Study:

  • To develop a method for detecting and characterizing low-concentration, non-enzymatic post-translational modifications in collagen.
  • To investigate the impact of specific physiological conditions (high fructose diet, hypertriglyceridemia) on collagen.
  • To establish a quantitative and qualitative analysis of protein alterations using peptide profiling.

Main Methods:

Related Experiment Videos

  • Deep enzymatic fragmentation of collagen (types I and III) using bacterial collagenase.
  • Capillary electrophoresis in acidic sodium phosphate buffer (pH 2.5) for peptide separation.
  • Statistical analysis including t-tests and principal component analysis (PCA) for profile interpretation.

    • Main Results:

    • The method successfully separated over 60 peptide peaks, revealing 2 to 13 changes in electrophoretic profiles.
    • Statistical analysis identified specific sections of electropherograms correlating with physiological states.
    • Qualitative differences in collagen modification were observed across the four investigated physiological regimes.

    Conclusions:

    • Deep enzymatic fragmentation coupled with capillary electrophoresis is effective for detecting subtle protein modifications.
    • This approach allows for the assessment of physiological condition impacts on protein structure.
    • The methodology provides a foundation for targeted pre-separation strategies in complex peptide mixtures.