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Related Experiment Videos

Hepatic tissue engineering on 3-dimensional biodegradable polymers within a pulsatile flow bioreactor.

Török E1, J M Pollok, P X Ma

  • 1Department of Hepatobiliary Surgery, University of Hamburg, Germany. toeroek@uke.uni-hamburg.de

Digestive Surgery
|July 21, 2001
PubMed
Summary

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Tissue engineering using hepatocyte spheroids on biodegradable poly-L-lactic acid (PLLA) in a flow bioreactor shows promise for liver transplantation. This method enhances cell function and creates a transplantable neotissue for in vivo liver support.

Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Hepatology

Background:

  • Optimal hepatocyte transplantation methods are under investigation.
  • Tissue engineering principles, including in vitro conditioning, are explored to enhance cell function.
  • Forming hepatocyte spheroids may increase cell mass and function for organ replacement.

Purpose of the Study:

  • To evaluate the formation and function of hepatocyte spheroids.
  • To assess the efficacy of using biodegradable poly-L-lactic acid (PLLA) in a flow bioreactor for hepatocyte conditioning.
  • To determine the potential of engineered hepatocyte spheroids as a substitute for lost liver function.

Main Methods:

  • Rat hepatocytes were seeded onto PLLA discs at 10 x 10(6) cells/ml.
  • Cultures were maintained in a flow bioreactor (24 ml/min) for 1, 2, 4, and 6 days.

Related Experiment Videos

  • Spheroid formation, morphology, and albumin production were analyzed.

    • Main Results:

    • Viable hepatocyte spheroids (50-200 microm) formed within 2 days.
    • Spheroid number and diameter stabilized by day 6.
    • Hepatocyte-specific albumin production was sustained throughout the culture period.

    Conclusions:

    • Short-term (2-3 days) conditioning of hepatocytes on PLLA in a flow bioreactor yields spheroids with liver-like morphology.
    • These engineered hepatocyte spheroids maintain metabolic function.
    • Hepatocyte spheroids represent a potential transplantable neotissue for in vivo liver support.