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Updated: Jul 31, 2026

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
Published on: December 27, 2016
Apoptosis in neuroblastomas induced by interferon-gamma involves the CD95/CD95L pathway
M Annicchiarico-Petruzzelli1, F Bernassola, P E Lovat
1IDI-IRCCS, Biochemistry Lab, University of Rome Tor Vergata, Italy.
Interferon-gamma (IFNγ) modulates CD95/CD95L expression and triggers apoptosis in neuroblastoma cells, suggesting IFNγ as a potential therapeutic agent for neuroblastoma (NB).
Area of Science:
- Molecular Biology
- Immunology
- Oncology
Background:
- The CD95/CD95 ligand (CD95L) system regulates apoptosis.
- Neuroblastoma (NB) is a pediatric cancer with a significant unmet need for effective therapies.
Purpose of the Study:
- To investigate the role of the CD95/CD95L system in human neuroblastoma (NB) cells.
- To evaluate the effect of interferon-gamma (IFNγ) on CD95/CD95L expression and apoptosis in NB cells.
Main Methods:
- Investigated constitutive and IFNγ-induced expression of CD95 and CD95L in the SK-N-BE(2) cell line.
- Assessed CD95-mediated apoptosis in response to IFNγ stimulation.
Main Results:
- IFNγ modulated the expression of CD95 and CD95L in NB cells.
- IFNγ triggered an autocrine apoptotic mechanism in NB cells, mediated by the CD95 system.
Conclusions:
- Modulation of CD95/CD95L expression and induction of apoptosis by IFNγ suggest its potential as a therapeutic agent for NB.
- Different molecular mechanisms, including retinoids via tTG and NMDA/gp120 via NOS, indicate diverse pathways for apoptosis induction in NB.
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