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Decreased wild-type full-length Et-A and -B receptors in neuroblastoma and Ewing sarcoma cells
P A Berry1, Y F Zhang, N D Carter
1ICRF Cancer Medicine Research Unit, St. James's University Hospital, Leeds, United Kingdom.
Background:
Endothelins and their receptors, Et-A and Et-B, play an essential role in differentiation and migration of neural crest cells. Expression of endothelin receptors has been examined in neuroblastoma and Ewing sarcoma cell lines.
Procedure:
RNA was amplified for Et-A and Et-B by RT-PCR. Amplified products were cloned into the expression vector pLNCX, which was used to transfect CHO cells. Binding characteristics of transfected CHO cells were examined.
Results:
Full-length Et-A mRNA was identified in all cell lines, in addition to a truncated Et-A product. CHO cells expressing full-length Et-A bound to endothelin, but cells expressing truncated Et-A did not. Full length Et-B mRNA was not detected, but two smaller molecular weight products were amplified. These are as yet uncharacterised.
Conclusions:
These results suggest that endothelins and their receptors may be important in the development and biology of neuroblastoma and Ewing sarcoma.
