Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A "cytotoxic" factor released from unactivated human lymphocytes.

M T Szabó, F Antoni

    Acta Biochimica Et Biophysica; Academiae Scientiarum Hungaricae
    |January 1, 1975
    PubMed
    Summary

    A novel protein mixture, LTx, released from human lymphocytes, demonstrates toxicity to target cells. LTx inhibits amino acid incorporation and increases chromium release, indicating cell damage.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Stabilities of neutral and basic esters of bendamustine in plasma compared to the parent compound: kinetic investigations by HPLC.

    Journal of pharmaceutical and biomedical analysis·2014
    Same author

    Towards optimization of time modulated chemical vapour deposition for nanostructured diamond films on Ti6Al4V.

    Journal of nanoscience and nanotechnology·2010
    Same author

    [Schizophrenia and the corpus callosum: morphologic and functional approach].

    L'Encephale·1996
    Same author

    Endotoxin inhibits glucuronidation in the liver. An effect mediated by intercellular communication.

    Biochemical pharmacology·1995
    Same author

    Net glucose production from acetone in isolated murine hepatocytes. The effect of different pretreatments of mice.

    The International journal of biochemistry·1994
    Same author

    Formation of leucyl-leucine-O-methylester in leucine-O-methylester treated cells.

    Acta physiologica Hungarica·1994

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Lymphocytes play a crucial role in immune responses.
    • Understanding lymphocyte-derived factors is key to elucidating immune mechanisms.

    Purpose of the Study:

    • To characterize a protein mixture (LTx) released from human tonsillar lymphocytes.
    • To investigate the cytotoxic effects of LTx on target cells.

    Main Methods:

    • Incubation of human tonsillar lymphocytes in individual and mixed cultures at 37°C.
    • Measurement of 14C-labelled amino acid incorporation in target lymphocytes.
    • Quantification of 51Cr release from labelled target cells.
    • Assessment of LTx binding to target cells.

    Main Results:

    • A protein mixture, LTx, was released from lymphocytes.
    • LTx significantly decreased amino acid incorporation in target cells.
    • LTx increased 51Cr release from target cells, indicating cell lysis.
    • Target cells exhibited binding of the LTx protein.

    Conclusions:

    • LTx is a cytotoxic protein released by human lymphocytes.
    • LTx induces cell damage through mechanisms involving reduced amino acid uptake and increased cell membrane permeability.
    • The binding of LTx to target cells suggests a direct mechanism of action.

    Related Experiment Videos