Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA tags.

J P Clewley1

  • 1Virus Reference Division, PHLS Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT. jclewley@phls.org.uk

Communicable Disease and Public Health
|July 27, 2001
PubMed
Summary
This summary is machine-generated.

New amplification methods enable sensitive protein detection using DNA tags. Antibodies coupled with DNA tags can identify protein antigens through DNA amplification, improving diagnostic tests.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic strategy used to establish etiologies of encephalitis in a prospective cohort of patients in England.

Journal of clinical microbiology·2011
Same author

DNA amplification : general concepts and methods.

Methods in molecular medicine·2011
Same author

Causality in acute encephalitis: defining aetiologies.

Epidemiology and infection·2010
Same author

Implications for HIV testing policy derived from combining data on voluntary confidential testing with viral sequences and serological analyses.

Sexually transmitted infections·2008
Same author

Genetic variants of parvovirus B19 identified in the United Kingdom: implications for diagnostic testing.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology·2006
Same authorSame journal

Biotechnology and microbiology--have reached the end of the line?

Communicable disease and public health·2005

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Current protein detection methods have limitations in sensitivity.
  • Detecting minute protein quantities is crucial for early disease diagnosis and research.

Purpose of the Study:

  • To explore the use of DNA amplification for enhanced protein detection.
  • To develop a novel assay for detecting protein antigens using DNA-tagged antibodies.

Main Methods:

  • Coupling DNA tags to antibodies.
  • Utilizing the antibody-DNA tag complex for protein antigen detection.
  • Employing DNA amplification techniques to signal the presence of the antigen.

Main Results:

  • Demonstrated the feasibility of detecting small protein amounts.

Related Experiment Videos

  • Showcased the specificity of DNA-tagged antibodies in antigen recognition.
  • Amplification of the DNA tag provided a measurable signal for protein presence.

    • Conclusions:

    • DNA amplification offers a powerful strategy for highly sensitive protein detection.
    • This approach holds potential for developing advanced diagnostic tools.
    • The DNA-tagged antibody method provides a versatile platform for biomarker discovery.