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Related Concept Videos

PCR01:32

PCR

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PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

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Related Experiment Video

Updated: Jun 3, 2026

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
07:35

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

DNA amplification : general concepts and methods.

N A Saunders1, J P Clewley

  • 1Molecular Biology Unit, Central Public Health Laboratory, London, UK.

Methods in Molecular Medicine
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

The polymerase chain reaction (PCR), a versatile DNA amplification technique, is increasingly used in diagnostic microbiology. PCR offers applications in sequencing, typing, and epidemiology, complementing traditional tests.

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Last Updated: Jun 3, 2026

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
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Published on: June 14, 2021

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Published on: May 22, 2012

Area of Science:

  • Molecular Biology
  • Biotechnology

Background:

  • The polymerase chain reaction (PCR) was first described in 1985.
  • PCR is a highly versatile DNA amplification method.

Purpose of the Study:

  • To highlight the versatility and applications of PCR.
  • To discuss the integration of PCR into diagnostic microbiology.

Main Methods:

  • The abstract discusses the general principles and applications of PCR.
  • It mentions the availability of PCR in kit form (AMPLICOR™).

Main Results:

  • PCR products can be readily sequenced for various diagnostic purposes.
  • PCR is becoming an adjunct to culture and serologic tests in diagnostic microbiology.

Conclusions:

  • PCR is a powerful and versatile tool for DNA amplification.
  • Its applications in diagnostics, typing, and epidemiology are expanding.