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Sodium Taurocholate Induced Severe Acute Pancreatitis in C57BL/6 Mice
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Platelet activating factor antagonism reduces the systemic inflammatory response in a murine model of acute

J S Lane1, K E Todd, B Gloor

  • 1Department of Surgery, UCLA Medical Center, Los Angeles, CA 90095, USA.

The Journal of Surgical Research
|July 27, 2001
PubMed
Abstract

Insights

Platelet activating factor (PAF) antagonism with Lexipafant reduced systemic inflammation in a mouse model of mild acute pancreatitis (AP). This suggests Lexipafant may treat mild AP after symptom onset.

Area of Science:

  • Gastroenterology
  • Inflammation research
  • Pharmacology

Background:

  • Platelet activating factor (PAF) antagonists, like Lexipafant, have been explored for treating severe acute pancreatitis (AP).
  • Investigating Lexipafant's efficacy in a murine model of mild, edematous AP is crucial for understanding its therapeutic potential.

Purpose of the Study:

  • To evaluate the effect of Lexipafant on local and systemic inflammatory markers in mild AP.
  • To determine if therapeutic administration of Lexipafant can mitigate the severity of acute pancreatitis.

Main Methods:

  • A murine model of mild acute pancreatitis was induced using cerulein.
  • Mice received either saline or Lexipafant treatment post-induction.
  • Serum cytokine levels, amylase activity, and lung myeloperoxidase (MPO) activity were measured.

Main Results:

  • PAF antagonism significantly reduced serum cytokines (TNF-alpha, IL-1beta), serum amylase, and lung MPO activity.
  • Histological analysis indicated a non-significant trend towards improvement with Lexipafant treatment.

Conclusions:

  • Therapeutic administration of Lexipafant effectively reduces systemic inflammation in mild acute pancreatitis in mice.
  • These findings support the potential utility of Lexipafant for treating mild pancreatitis following clinical manifestation.

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