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Updated: Aug 6, 2026
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Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
Published on: December 28, 2017
A DNA polymorphism specific to Candida albicans strains exceptionally successful as human pathogens
L Giblin1, A Edelmann, N Zhang
1Institute of Molecular BioSciences, College of Sciences, Massey University, Palmerston North, New Zealand.
Gene
|July 27, 2001
Summary
A specific DNA fragment, MU13-4, distinguishes the successful pathogenic genotype of Candida albicans. This discovery aids in differentiating strains and understanding their role in infections.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- A significant proportion of pathogenic Candida albicans isolates belong to a common genotype, identified by the Ca3 sequence.
- The high prevalence of this genotype suggests underlying genetic factors contributing to its success as a pathogen.
Purpose of the Study:
- To identify genetic determinants specific to the general-purpose genotype of Candida albicans.
- To develop methods for distinguishing between general-purpose and other C. albicans strains.
Main Methods:
- Genome comparison of general-purpose genotype strains with other C. albicans strains.
- Identification and characterization of a specific DNA fragment (MU13-4) using hybridization and Southern blotting.
- Bioinformatic analysis using the Stanford C. albicans genome database.
Main Results:
- A 985 bp HpaII fragment (MU13-4) was identified, present in 90% of general-purpose genotype strains but only 10% of others.
- MU13-4 contains repetitive DNA and is dispersed in general-purpose strains, but rarer or absent in others.
- The polymorphism is likely due to a deletion in non-cluster strains.
Conclusions:
- Specific DNA polymorphisms can effectively differentiate general-purpose genotype strains of Candida albicans.
- The identified fragment (MU13-4) can be used to develop PCR-based diagnostic tools.
- This facilitates research into the differential impact of C. albicans genotypes on disease outcomes.
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