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Related Concept Videos

Recombinant DNA01:09

Recombinant DNA

Overview
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Recombinant DNA01:09

Recombinant DNA

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DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
The Central Dogma01:20

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...

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Related Experiment Video

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Recombineering Homologous Recombination Constructs in Drosophila
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Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

Introductory experiments in recombinant DNA.

R C Tait

    Current Issues in Molecular Biology
    |July 27, 2001
    PubMed
    Summary
    This summary is machine-generated.

    This resource uses nine practical exercises to teach fundamental recombinant DNA principles. It clarifies that DNA represents genes and alterations in DNA lead to changes in genetic traits, aiding molecular biology education.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Biotechnology

    Background:

    • Understanding DNA and gene function is crucial in life sciences.
    • Molecular biology principles underpin many advancements in medicine and biotechnology.
    • Effective teaching resources are needed to explain complex genetic concepts.

    Purpose of the Study:

    • To provide a practical teaching resource for recombinant DNA technology.
    • To illustrate the relationship between DNA, genes, and genetic properties.
    • To support the education of students and professionals in life sciences.

    Main Methods:

    • Development of nine hands-on laboratory exercises.
    • Focus on demonstrating basic principles of recombinant DNA.
    • Explanation of DNA as the basis of genes and heritability.

    Main Results:

    • Practical exercises effectively demonstrate core recombinant DNA concepts.
    • The resource clarifies the link between DNA structure and genetic traits.
    • The exercises are designed for diverse learners, from high school to professional levels.

    Conclusions:

    • The developed exercises serve as a valuable tool for teaching molecular biology.
    • This resource enhances understanding of DNA technology and its applications.
    • It bridges the gap between theoretical knowledge and practical application in genetics.