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[Effects of composite blood-activating decoction on bone marrow microenvironment in mice of immune-induced aplastic
Objective:
To explore the mechanism of elevating efficacy for aplastic anemia (AA) by using blood-activating and stasis-eliminating drugs.
Methods:
Immune-induced aplastic anemia model was established. Each mouse was gastrogavaged by 0.2 ml 100% composite blood-activating decoction (CBAD) twice a day. On the 10th day, the bone marrow histology, CFU-F, adhesive function of the cultured stromal cell layer, bone marrow PO2 were observed.
Results:
In CBAD group, the WBC count, bone marrow karyocytes, bone marrow hematopoietic tissue volume, CFU-F count were significantly higher than those in AA group (P < 0.01). Moreover, the adhesive function of stromal cells and bone marrow PO2 recovered to normal level.
Conclusion:
The commonly used composite blood-activating decoction could promote the recovery and oxygen-supply of bone marrow microenvironment in AA mice, and improved the bone marrow hematopoiesis.
