Enzyme modified microband electrodes: cross-talk effects and their elimination

Area of Science:

• Electrochemistry
• Biosensor Technology
• Biomedical Engineering

Background:

• Microband electrodes offer high surface area for biosensing applications.
• Electrochemical immobilization is a key technique for enzyme-based biosensor development.
• Minimizing signal interference between adjacent sensing elements is crucial for multianalyte devices.

Purpose of the Study:

• To develop a stable microbiosensor for glucose detection.
• To investigate and mitigate electrochemical cross-talk between microbands.
• To assess the feasibility of creating a multianalyte microbiosensor.

Main Methods:

• Direct electrochemically assisted immobilization of glucose oxidase onto a microband electrode.
• Fabrication of a four-microband electrode array with specific dimensions.
• Evaluation of cross-talk elimination strategies, including catalase deposition.

Main Results:

• A stable glucose oxidase microbiosensor was achieved with a Michaelis-Menten constant of 12 mM and a maximum current of 80 nA.
• Catalase deposition on adjacent microbands proved to be the most effective method for reducing cross-talk.
• Signal interference on unmodified microbands was reduced to approximately 3%.

Conclusions:

• The developed method enables the creation of stable and reliable glucose microbiosensors.
• Effective cross-talk suppression using catalase is demonstrated, paving the way for advanced biosensor designs.
• This approach holds significant potential for the development of multianalyte microbiosensors.