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Microcontact Printing of Proteins for Cell Biology
Published on: December 5, 2008
Investigation of microcontact transfer of proteins from a selectively plasma treated elastomer stamp by fluorescence
X Feng1, C J Roberts, D A Armitage
1Department of Pharmaceutical Sciences, The University of Nottingham, UK.
The Analyst
|August 2, 2001
Abstract:
Selective plasma treatment of the recessed regions of the elastomer stamps is shown to alter the resultant protein patterns. Fluorescence microscopy is demonstrated to be an excellent tool to discriminate between regions of microcontact printed fluorescent dye-labelled albumin in polystyrene. Atomic force microscopy and shear force microscopy are used to provide high-resolution images of the patterned protein layers. The formation and characteristics of the patterns formed by these alternative strategies is discussed.

