[The biological effects of recombinant human osteogenic protein-1 to cultured pulp cells]

The study was to observe the biological effects of rhOP-1 on the proliferation, ALPase activity and osteocalcin of dental pulp cells by cell culture technique, MTT, enzyme kinetic methods and radioimmunoassay. The results indicated rhOP-1 could obviously promote the proliferation and enhanced ALPase activity and osteocalcin secretion of pulp cells with showing dose-dependent relationship in this experimental dose range. Thus, rhOP-1 can induce dental pulp cells to differentiate the cells expressing osteoblast phenotype.