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Interactions between A-9THC and capsaicin on isolated lamb bladder detrusor
C Bartocci1, M G Evandri, P Tucci
1Department of Pharmacology and Physiology, School of Pharmacy, University of Rome La Sapienza, Italy.
Farmaco (Societa Chimica Italiana : 1989)
|August 3, 2001
Summary
Cannabinoid delta-9-tetrahydrocannabinol (delta-9THC) altered isolated lamb detrusor responses to capsaicin, shifting relaxation to contraction. This suggests delta-9THC may inhibit CGRP release, impacting bladder function.
Area of Science:
- Pharmacology
- Urology
- Neuroscience
Background:
- Capsaicin affects bladder tissue via VR1 receptors, typically causing relaxation in lambs mediated by CGRP.
- Endogenous cannabinoids like anandamide also interact with VR1 receptors, potentially releasing CGRP.
Purpose of the Study:
- To investigate if delta-9-tetrahydrocannabinol (delta-9THC) modifies the response of isolated lamb detrusor muscle to capsaicin.
Main Methods:
- Isolated lamb detrusor tissues were exposed to varying concentrations of delta-9THC.
- Tissues were then stimulated with capsaicin, and responses were recorded.
- The effect of atropine on capsaicin-induced contractions post-delta-9THC treatment was assessed.
Main Results:
- Delta-9THC alone did not affect detrusor tissues at tested concentrations.
- Following delta-9THC, capsaicin induced contractions in most tissues, an effect blocked by atropine.
- This suggests delta-9THC may inhibit capsaicin-induced CGRP release, unmasking a contractile response.
Conclusions:
- Delta-9THC appears to alter the balance of signaling pathways in the lamb detrusor.
- By potentially inhibiting CGRP release via cannabinoid receptors, delta-9THC may shift the bladder response from relaxation to contraction.