Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro reconstitution of the end replication problem.

R Ohki1, T Tsurimoto, F Ishikawa

  • 1Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.

Molecular and Cellular Biology
|August 4, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lattice dynamics and carrier recombination in GaAs/GaAsBi nanowires.

Scientific reports·2023
Same author

Polytypism in GaAs/GaNAs core-shell nanowires.

Nanotechnology·2020
Same author

Effects of growth temperature and thermal annealing on optical quality of GaNAs nanowires emitting in the near-infrared spectral range.

Nanotechnology·2019
Same author

Identification of a tumor-specific allo-HLA-restricted γδTCR.

Blood advances·2019
Same author

Superconductivity under pressure in the Dirac semimetal PdTe<sub>2</sub>.

Journal of physics. Condensed matter : an Institute of Physics journal·2019
Same author

Molecular beam epitaxial growth of dilute nitride GaNAs and GaInNAs nanowires.

Nanotechnology·2019

The end replication problem, which limits DNA replication at chromosome ends, is now biochemically demonstrated. This study shows lagging strand synthesis halts, creating 3' overhangs, confirming a long-standing hypothesis about DNA ends.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The end replication problem hypothesis suggests linear DNA ends are incompletely replicated during lagging strand synthesis.
  • This hypothesis is widely accepted to explain telomere attrition but lacks direct biochemical evidence.

Purpose of the Study:

  • To biochemically investigate the replication of linear DNA ends.
  • To formally demonstrate the end replication problem in vitro.

Main Methods:

  • Established a novel in vitro linear simian virus 40 DNA replication system.
  • Utilized biotin-labeled linear DNA conjugated to avidin-coated beads for replication.
  • Analyzed bead-bound replication products to assess DNA synthesis.

Main Results:

Related Experiment Videos

  • Demonstrated complete synthesis of the leading DNA strand to the end.
  • Showed lagging strand synthesis gradually halts in the terminal ~500 bp region.
  • Identified 3' overhangs resulting from incomplete lagging strand replication.

Conclusions:

  • Formally demonstrated the end replication problem through a biochemical approach.
  • Findings align with observations in telomerase-negative mammalian cells.
  • Provides a foundation for detailed telomere replication studies.