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Related Concept Videos

Multipotency of Hematopoietic Stem Cells01:19

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Embryonic Stem Cells00:58

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
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Normal stress is a type of stress that occurs when forces act perpendicular, or normal, to a material's cross-sectional area. This stress often arises in structures when subjected to axial loading, which is the application of force along the axis of an object. A practical example of this can be found in bridge truss members.
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Related Experiment Video

Updated: Jan 24, 2026

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
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Normal and leukemic CD34-negative human hematopoietic stem cells.

D Bonnet1

  • 1Laboratory of Molecular and Cellular Biology of Hematopoiesis, Coriell Institute for Medical Research, 401 Haddon Avenue, Camden, NJ 08103, USA. dbonnet@cimr.umdnj.edu

Reviews in Clinical and Experimental Hematology
|August 7, 2001
PubMed
Summary
This summary is machine-generated.

Hematopoietic stem cell purification has advanced, but the CD34 marker isn't unique. Recent findings reveal CD34- cells also possess repopulating capacity, challenging existing stem cell dogma.

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Area of Science:

  • Hematology
  • Stem Cell Biology
  • Immunology

Background:

  • Hematopoietic stem cells (HSCs) are crucial for blood formation and are purified using cell surface markers.
  • CD34, a sialomucin, has been the primary marker for primitive HSCs, decreasing with differentiation.
  • However, CD34 expression is not exclusive to HSCs, appearing on progenitors and endothelial cells.

Purpose of the Study:

  • To review the discovery of CD34- repopulating cells.
  • To examine the implications of CD34- cells in human hematopoiesis.
  • To discuss the clinical and scientific impact of these findings.

Main Methods:

  • Review of recent murine and human studies on stem cell purification.
  • Analysis of data on cell surface marker expression in hematopoietic stem cells.
  • Examination of multilineage repopulation capacity in CD34- and CD34+ cell populations.

Main Results:

  • Studies indicate that some stem cells capable of multilineage repopulation do not express detectable CD34.
  • This challenges the established view that all repopulating cells are CD34+.
  • The exact relationship between CD34- and CD34+ stem cells remains unclear.

Conclusions:

  • The existence of CD34- repopulating cells necessitates a re-evaluation of hematopoietic stem cell organization.
  • Clinical and experimental protocols may need adjustment to account for both CD34+ and CD34- stem cell populations.
  • Further research is required to fully understand the role and potential of CD34- stem cells.