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Caerulein-induced pancreatitis in Wistar rats
G Farrant1, F M Abu-Zidan, X Liu
1Pancreatitis Research Group, Department of Surgery, Faculty of Medicine and Health Science, University of Auckland, New Zealand.
Contemporary Topics in Laboratory Animal Science
|August 7, 2001
Summary
This study presents a refined rat model for studying pancreatitis using osmotic pumps. This improved method reliably induces mild pancreatitis in conscious rats with reduced handling and costs.
Area of Science:
- Veterinary Medicine
- Animal Models
- Pancreatitis Research
Background:
- Caerulein-induced pancreatitis is a common research model.
- Previous models often involve stressed or handled animals.
- A need exists for refined, cost-effective models.
Purpose of the Study:
- To describe a refined model of intravenous caerulein-induced pancreatitis.
- To utilize osmotic infusion pumps in conscious, unrestrained Wistar rats.
- To establish a reliable method for inducing mild edematous pancreatitis.
Main Methods:
- Intravenous infusion of caerulein using osmotic pumps.
- Utilizing Alzet implantable osmotic pumps primed with saline.
- Employing PE-55 catheter tubing for infusion in conscious, unrestrained Wistar rats.
Main Results:
- Reliably induced mild edematous pancreatitis.
- Histological verification of pancreatitis.
- Demonstrated advantages including unrestrained animals and reduced handling.
Conclusions:
- The refined model offers a reliable and efficient method for pancreatitis research.
- Advantages include reduced animal stress, decreased handling, and lower costs.
- This model is suitable for studying edematous pancreatitis in Wistar rats.