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Related Experiment Videos

Improved method for euglobulin clot lysis time.

M L Lowe, D C Cannon

    Clinical Biochemistry
    |June 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Technical improvements to the euglobulin clot lysis test enhance accuracy and sample stability. Storing plasma at minus 20 degrees ensures prolonged stability, with established normal ranges for citrated and oxalated samples.

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    Area of Science:

    • Hematology
    • Clinical Chemistry

    Background:

    • The euglobulin clot lysis test is crucial for assessing fibrinolytic activity.
    • Accurate endpoint determination and sample stability are critical for reliable test results.

    Purpose of the Study:

    • To introduce technical improvements for the euglobulin clot lysis test.
    • To define optimal conditions for plasma sample storage and determine normal ranges.

    Main Methods:

    • Utilized Evans Blue dye for improved endpoint determination.
    • Assessed plasma sample stability at various temperatures (4°C, 30°C, -20°C).
    • Established normal ranges for citrated and oxalated plasma.

    Main Results:

    • Evans Blue dye facilitates endpoint determination in the euglobulin clot lysis test.

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  • Plasma samples exhibit limited stability at 4°C and 30°C but are stable long-term at -20°C.
  • Normal ranges: >70 minutes for citrated plasma, >50 minutes for oxalated plasma.
  • Samples with accelerated clot lysis show reduced stability at 30°C.

    • Conclusions:

    • Technical refinements improve the euglobulin clot lysis assay.
    • Minus 20°C is the recommended storage temperature for plasma samples.
    • Established normal ranges provide a benchmark for fibrinolytic activity assessment.