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An improved method for plasma fibrinogen based on thrombin time measurement.
Summary
This study introduces a modified thrombin time method for measuring plasma fibrinogen, effectively eliminating heparin interference. The enhanced assay offers accurate and precise results, suitable for semi-automated analysis.
Area of Science:
- Clinical Chemistry
- Hematology
- Biochemistry
Background:
- Plasma fibrinogen measurement is crucial for assessing coagulation.
- Thrombin time-based methods are rapid but susceptible to heparin interference, causing falsely low results.
Purpose of the Study:
- To develop a modified thrombin time method for plasma fibrinogen quantification.
- To eliminate heparin interference in thrombin time assays.
- To validate the accuracy and precision of the modified method.
Main Methods:
- Incorporation of Polybrene into the buffer to neutralize heparin.
- Comparison with a reference method based on clottable protein.
- Assessment of day-to-day precision.
Main Results:
- The modified method effectively eliminated heparin interference.
- Excellent agreement was observed between the proposed method and the reference procedure.
- High day-to-day precision was demonstrated (C.V. 3.5%).
Conclusions:
- The Polybrene-modified thrombin time method provides a reliable and accurate measurement of plasma fibrinogen.
- This method is easily adaptable for semi-automated laboratory use.
- It offers a valuable alternative for routine fibrinogen determination, especially in patients receiving heparin therapy.