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Intraperitoneal blood exacerbates the remote inflammatory response to murine peritonitis

J L Johnson1, B H Wallace, C D Mareen

  • 1Department of Surgery, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA.

The Journal of Trauma
|August 9, 2001
PubMed
Abstract

Insights

Intra-abdominal blood, combined with inflammation, significantly amplifies the systemic inflammatory response and lung injury in mice. This amplified injury is linked to increased circulating and lung tissue chemokine levels.

Area of Science:

  • Inflammation and Immunology
  • Sepsis Pathophysiology
  • Hemorrhage and Organ Injury

Background:

  • Investigating the systemic inflammatory response to peritonitis.
  • Utilizing a murine model for hemorrhage, peritonitis, and multiple organ dysfunction syndrome.

Purpose of the Study:

  • To determine the effects of intra-abdominal blood on the systemic response to peritonitis.
  • To elucidate the role of chemokines in amplified lung injury.

Main Methods:

  • Male ICR mice subjected to hemorrhage and intraperitoneal zymosan.
  • Experimental group received intraperitoneal blood; control group did not.
  • Measured lung myeloperoxidase, edema, injury score, and chemokine production.

Main Results:

  • Peritoneal blood with inflammation increased lung neutrophil sequestration and weight.
  • Significantly higher plasma and lung tissue chemokine levels (KC, MCP-1, MIP-2) were observed.
  • Neutrophil chemoattractants correlated with myeloperoxidase and lung injury scores.

Conclusions:

  • Blood in the peritoneal cavity, when combined with inflammation, synergistically amplifies the systemic inflammatory response.
  • Amplified lung injury was associated with elevated circulating and lung tissue chemokine concentrations.
  • Findings highlight the complex interplay between hemorrhage, inflammation, and organ injury.

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