Related Experiment Video
Updated: Jul 16, 2026

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
Published on: June 25, 2010
A rapid and quantitative mass spectrometry method for determining the concentration of acylcarnitines and aminoacids
L Braida1, S Crovella, M Boniotto
1Servizio di Genetica Medica, IRCCS Burlo Garofolo, Via dell'Istria 5/1, 34137 Trieste, Italy.
Abstract:
We describe a quantitative, rapid, sensitive and reproducible tandem mass spectrometry (MSMS) method for the one-step detection of aminoacid (AAs) and acylcarnitine (ACs) concentrations in amniotic fluid. This technology is quicker and more sensitive than other methods used to date since it is possible to determine very low AA and AC concentrations in samples simultaneously in a single run. The high degree of automation allows a large number of pregnancies to be screened for metabolic defects in a very short time.

