Related Experiment Video
Updated: Aug 11, 2026
11:14
Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
Published on: January 6, 2017
The design of antiparallel coiled coils
1Department of Chemistry, Indiana University, 800 East Kirkwood Avenue, Bloomington, IN 47405-7102, USA. oakley@indiana.edu
Current Opinion in Structural Biology
|August 10, 2001
Summary
Antiparallel coiled coils are crucial in biology. Researchers designed model versions for protein studies, validating advanced protein design techniques with structural data.
Area of Science:
- Structural biology
- Protein design
- Biophysics
Background:
- Antiparallel coiled coils play significant roles in biological systems.
- Understanding their structure and interactions is key to protein science.
Purpose of the Study:
- To highlight the importance of antiparallel coiled coils through recent studies.
- To detail the design and application of model antiparallel coiled coils.
- To evaluate the success of current protein design methods using structural data.
Main Methods:
- Review of recent structural studies on antiparallel coiled coils.
- Design and characterization of model antiparallel coiled coil systems.
- Analysis of high-resolution structural data from designed helical bundles.
Main Results:
- Demonstrated the utility of designed antiparallel coiled coils for protein fragment reassembly.
- Provided insights into the energetic factors governing helix orientation specificity.
- Validated the accuracy of contemporary protein design strategies through structural analysis.
Conclusions:
- Antiparallel coiled coils are essential structural motifs in proteins.
- Designed coiled coils serve as valuable tools for fundamental biological research.
- Advanced protein design approaches are increasingly successful in creating functional protein structures.
Related Concept Videos
01:13Protein Organization
Overview
01:22Protein Folding
Overview
01:22Protein Folding
Overview
01:03Single-Strand DNA Binding Proteins
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
01:24Protein Organization
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
The primary structure of a protein is its amino acid sequence.
01:25Protein Folding
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...