Related Experiment Videos
In vitro evidence that immunuaffinity-purified MOG contains immunogenic quantities of contaminating mouse IgG;
T Ohashi1, M Yukitake, A Slavin
1Center for Neurologic Diseases, Laboratory of Molecular Immunology, Brigham and Women's Hospital and Harvard Medical School, 77 Avenue Louis Pasteur, HIM 786, Boston, MA 02115, USA.
Abstract:
Myelin oligodendrocyte glycoprotein (MOG) has attracted considerable attention due to its possible role in multiple sclerosis (MS). Previous studies have shown that MOG-reactive T cells are more commonly detected in MS patients than controls. In this report, we studied human MOG-reactive T cell clones generated from healthy individuals as well as MS patients. Our results indicate that immunoaffinity-purified MOG, which was routinely used in prior studies, is contaminated by anti-MOG antibody (mouse IgG), despite the inability to detect IgG by Western blotting. Here, we used continuous SDS-PAGE, which enabled us to isolate highly purified MOG devoid of contaminating mouse IgG.