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Related Experiment Videos

[Development and applications of high performance membrane chromatography].

J Wang1, Z Meng, L Zhou

  • 1Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116012.

Se Pu = Chinese Journal of Chromatography
|August 14, 2001
PubMed
Summary

High performance membrane chromatography (HPMC) offers advanced biomolecule separation. This review covers base membranes, geometries, and applications in affinity, ion-exchange, and hydrophobic interaction chromatography, with future enantiomeric separation prospects.

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Area of Science:

  • Biochemical Engineering
  • Separation Science

Context:

  • High performance membrane chromatography (HPMC) is a rapidly advancing separation technique.
  • Biomolecule purification and analysis are critical in biotechnology and pharmaceuticals.

Purpose:

  • To introduce the development and applications of HPMC.
  • To review base membranes, geometries, and separation modes including HPAMC, HPIEMC, and HPHIMC.
  • To prospect the use of HPMC for enantiomeric separations.

Summary:

  • This work reviews base membranes and geometries for HPMC.
  • It details separations of biomolecules using high performance affinity membrane chromatography (HPAMC), high performance ion-exchange membrane chromatography (HPIEMC), and high performance hydrophobic interaction membrane chromatography (HPHIMC).

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  • Future applications of HPMC for enantiomeric separation are discussed.

    • Impact:

    • Provides a comprehensive overview of HPMC for researchers and practitioners.
    • Highlights the versatility of HPMC in various biomolecule separation applications.
    • Identifies potential future directions for HPMC, particularly in enantiomeric separations.