Standardization of housedust allergen in human skin test trials

Standardization was carried out by comparison of Bencard and our own ('Human') housedust allergens in human skin test trials. The dose response curves on hypersensitive patients were parallel, linear, and there was no difference between preparations according to the variance analysis. The relative potency was 1.33 compared to the Bencard allergen. 95% confidence limits were 0.29-2.37. The correlation coefficient evaluated from all data was 0.90. Our experience suggests that human skin test trials seem to be a simple and convenient method for the standardization of housedust allergens. According to the results of analysis of factorials, data suitable for standardization can be obtained if the area of both the wheal and flare as different parameters are recorded 10 and/or 20 minutes after the intradermal injections.