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Lymphocyte blastogenesis studied by volume spectroscopy.

H B Steen, V Nielsen

    Scandinavian Journal of Immunology
    |January 1, 1979
    PubMed
    Summary
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    This study used volume spectroscopy to track human lymphocyte blastogenesis in response to concanavalin A (Con A). Cell and nuclear growth dynamics were observed before detectable DNA synthesis, offering insights into immune response assays.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biophysics

    Background:

    • Human blood lymphocyte blastogenesis is a key indicator of immune response.
    • Concanavalin A (Con A) is a widely used mitogen to stimulate lymphocytes.
    • Conventional assays for immune response may have limitations.

    Purpose of the Study:

    • To investigate human lymphocyte blastogenesis using volume spectroscopy.
    • To characterize cellular and nuclear volume changes during Con A stimulation.
    • To compare volume spectroscopy with traditional immune response assays.

    Main Methods:

    • Modified Coulter counter used for measuring cellular and nuclear volumes.
    • Leukocyte cultures stimulated with concanavalin A (Con A).
    • Cell aggregation addressed using alpha-methyl-mannoside and EDTA.

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    Main Results:

    • Cellular growth began 8-12 hours post-Con A stimulation, preceding nuclear growth by 6 hours.
    • Nuclear growth initiated 6-8 hours before increased 3H-thymidine incorporation.
    • Cellular volume increased ~4-fold and nuclear volume ~3-fold before mitosis.
    • Increased cell volume response observed even at supraoptimal Con A concentrations.

    Conclusions:

    • Volume spectroscopy provides detailed insights into lymphocyte blastogenesis.
    • Cellular and nuclear volume changes precede detectable DNA synthesis.
    • This method offers potential advantages over conventional immune response assays.