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Related Experiment Videos

Deletrious effect of prolonged cold ischemia on renal function.

B M Stubenitsky1, L Brasile, M H Booster

  • 1Department of Surgery, AZM University Hospital, P. Debyelaan 25, 6202 Maastricht, The Netherlands. nitsky@hotmail.com

Transplant International : Official Journal of the European Society for Organ Transplantation
|August 21, 2001
PubMed
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Prolonged cold ischemia (CI) in kidneys impairs function after 24 hours, preceding cell death at 48 hours. This study models CI injury using ex-vivo renal metabolism and function assessment before reperfusion.

Area of Science:

  • Nephrology
  • Transplantation Biology
  • Organ Preservation

Background:

  • Prolonged cold ischemia (CI) negatively impacts kidney transplant outcomes.
  • The precise cellular effects of CI on kidney function remain incompletely understood.

Purpose of the Study:

  • To establish a model for identifying CI-induced kidney injury.
  • To evaluate ex-vivo renal metabolism and function before reperfusion following varying CI durations.

Main Methods:

  • Small bovine kidneys underwent cold storage (24, 48, 72, 96 hours) in Viaspan.
  • Kidneys were subsequently warm-perfused (32°C) using Exsanguineous Metabolic Support (EMS) technology.
  • Evaluated parameters included oxidative metabolism, vascular resistance, diuresis, and glomerular filtration rate.

Related Experiment Videos

Main Results:

  • Kidney function, including oxygen consumption and diuresis, remained stable up to 48 hours of CI.
  • After 72 and 96 hours of CI, increased vascular resistance and reduced oxygen consumption and diuresis were observed (P < 0.05).
  • Glomerular filtration rate significantly decreased after more than 24 hours of CI (P < 0.05).

Conclusions:

  • Kidney function is compromised after 24 hours of CI.
  • Functional decline precedes the loss of cellular viability, which occurs after 48 hours of CI.
  • The developed ex-vivo model effectively identifies CI-induced kidney injury.