Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rational selection and quantitative evaluation of antisense oligonucleotides.

A Jayaraman1, S P Walton, M L Yarmush

  • 1Center for Engineering in Medicine/Surgical Services, Massachusetts General Hospital, Boston 02114, USA.

Biochimica Et Biophysica Acta
|August 22, 2001
PubMed
Summary

Developing effective antisense oligonucleotides (ASOs) for gene expression modulation is challenging. A new prediction algorithm accurately identifies potent ASOs, significantly aiding therapeutic development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response: Use of the WISCI-II score in assessing outcome of intensive robot-assisted gait training in spinal cord injury.

Spinal cord·2024
Same author

Aryl hydrocarbon receptor activity in intestinal epithelial cells in the formation of colonic tertiary lymphoid tissues.

American journal of physiology. Gastrointestinal and liver physiology·2024
Same author

Quantum bath suppression in a superconducting circuit by immersion cooling.

Nature communications·2023
Same author

Cost effectiveness of a community based prevention and treatment of acute malnutrition programme in Mumbai slums, India.

PloS one·2018
Same author

Automated end-to-end blood testing at the point-of-care: Integration of robotic phlebotomy with downstream sample processing.

Technology·2018
Same author

Historical and Projected Surface Temperature over India during the 20<sup>th</sup> and 21<sup>st</sup> century.

Scientific reports·2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Antisense oligonucleotides (ASOs) offer therapeutic potential for modulating gene expression.
  • Selecting effective ASOs and quantifying their efficacy remain significant challenges in drug discovery.
  • Existing methods for ASO selection lack quantitative assessment of potency and specificity.

Purpose of the Study:

  • To evaluate the antisense activity of rationally selected ASOs based on a previously developed prediction algorithm.
  • To validate the algorithm's ability to identify high-affinity and effective ASOs against target messenger RNAs (mRNAs).
  • To assess the efficacy of ASOs across different target genes and chemical modifications.

Main Methods:

  • Rational selection of ASOs using a prediction algorithm based on mRNA-oligonucleotide hybridization affinity.

Related Experiment Videos

  • In vitro testing of selected ASOs against three model target mRNAs (human lactate dehydrogenase A and B, rat gp130) in cell culture.
  • Quantitative assessment of ASO efficacy using kinetic PCR to measure target mRNA reduction via RNase H recruitment.

    • Main Results:

    • ASOs predicted to have high target affinity demonstrated effectiveness in almost all tested cases.
    • Successful gene silencing was observed across three distinct mRNA targets and two cell types.
    • Both phosphodiester and phosphorothioate oligonucleotide chemistries proved effective with the rationally selected ASOs.

    Conclusions:

    • The prediction algorithm effectively identifies potent and specific antisense oligonucleotides.
    • This rational selection approach significantly enhances the discovery of effective ASOs for therapeutic applications.
    • The validated method holds promise for developing ASOs targeting various diseases through specific gene expression modulation.