Automated constant-current coulometric assay system for ascorbic acid and sodium ascorbate.

Area of Science:

• Analytical Chemistry
• Electrochemistry

Background:

• Ascorbic acid and sodium ascorbate are vital compounds with significant analytical requirements.
• Automated analytical systems offer enhanced efficiency and reliability in chemical assays.

Purpose of the Study:

• To evaluate the performance of an automated constant-current coulometric system for quantifying ascorbic acid and sodium ascorbate.
• To determine the accuracy and precision of the automated system under optimized electrochemical conditions.

Main Methods:

• Utilized a constant-current coulometric titration system for automated sample analysis.
• Optimized electrochemical parameters to ascertain optimal assay conditions.
• Evaluated system performance based on accuracy and precision metrics.

Main Results:

• The automated system demonstrated capability for unattended analysis of 25 samples over 2.5 hours.
• Optimized conditions yielded high accuracy and precision, with results within +/- 0.3% (95% confidence).

Conclusions:

• The described automated coulometric system provides a reliable and efficient method for the assay of ascorbic acid and sodium ascorbate.
• The system's high accuracy and precision make it suitable for routine quality control and research applications.