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Updated: Jul 29, 2026
10:32
On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Published on: March 2, 2012
Ion chromatography on-chip.
J P Murrihy1, M C Breadmore, A Tan
1Department of Chemistry, University College Cork, Ireland.
Journal of Chromatography. A
|August 28, 2001
Summary
This study demonstrates on-chip separation of inorganic anions using ion-exchange chromatography. Novel quaternary ammonium latex particles enable efficient separation in microchannels, advancing analytical chemistry.
Area of Science:
- Analytical Chemistry
- Microfluidics
- Separation Science
Background:
- Ion-exchange chromatography is a key separation technique.
- Microfluidic devices offer advantages for analytical separations.
- Developing efficient stationary phases for on-chip chromatography is crucial.
Purpose of the Study:
- To develop an on-chip ion-exchange chromatography system for inorganic anion separation.
- To investigate the use of quaternary ammonium latex particles as a stationary phase.
- To evaluate the performance of the microfluidic device for anion analysis.
Main Methods:
- Fabrication of micro separation channels on a silicon wafer using photolithography and etching.
- Coating of channels with quaternary ammonium latex particles.
- On-chip separation of anions (nitrite, nitrate, iodide, thiourea) with off-chip injection and UV detection.
- Optimization of channel dimensions and eluent concentration.
Main Results:
- Successful on-chip separation of nitrite, nitrate, iodide, and thiourea.
- Demonstrated enhanced analyte-stationary phase interactions due to narrow channel depths (0.5-10 microm).
- Achieved linear ranges from 5 to 1000 microM for nitrite and nitrate with detection limits of 0.5 microM.
Conclusions:
- On-chip ion-exchange chromatography is feasible for inorganic anion separation.
- Quaternary ammonium latex particles are effective stationary phases for microfluidic anion analysis.
- The developed system shows promise for sensitive and efficient on-chip separation of anions.
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