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Separation of antithrombin III variants by micellar electrokinetic chromatography
R Dönges1, J Römisch, H Stauss
1Aventis Pharma, Quality Operations, Marburg, Germany. reiner.doenges@aventis.com
Abstract:
The characterisation of proteins is still one of the most challenging analytical tasks in modern bioanalysis. Due to the complex structure of proteins, several analytical techniques are often required to get sufficient information. Antithrombin III (AT III), a high-molecular-mass plasma glycoprotein which is an important protease inhibitor and the main modulator of thrombin activity, circulates in plasma in two isoforms, the so-called AT III-alpha (90-95%) and -beta (5-10%). Micellar electrokinetic chromatography was used to analytically separate these AT III variants, which differ in their affinity to the polysaccharide heparin.