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Region-specific decrease in 5-HT1A and 5-HT1B binding sites after intra-hippocampal ibotenic acid injections in the
D Aït Amara1, L Segu, M C Buhot
1INSERM U.488 Stéroïdes et Système Nerveux, 80 rue du Général Leclerc, 94276, Bicêtre, France.
Abstract:
The cellular location of 5-HT1A and 5-HT1B binding sites in the hippocampus was investigated using a stereotaxic unilateral lesion of the CA1 field by ibotenic acid, followed by autoradiography on brain sections with the specific ligands [3H]8-OH-DPAT and S-CM-G[125I]TNH2, respectively. As compared to the contralateral side of the lesion, the ipsilateral side exhibited a decrease in the density of 5-HT1A binding sites in the strata oriens and radiatum of CA1, and of 5-HT1B binding sites in the dorsal subiculum and stratum oriens of CA1. The results demonstrate that 5-HT1A binding sites are located on dendrites whereas 5-HT1B binding sites are located on axon terminals of CA1 pyramidal cells.
