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Cryoloop vitrification yields superior survival of Rhesus monkey blastocysts.
R R Yeoman1, B Gerami-Naini, S Mitalipov
1Andrology/Embryology Laboratory, Department of Obstetrics/Gynecology, Oregon Health Sciences University, Portland, Oregon 97201, USA. yeomanr@ohsu.edu
Human Reproduction (Oxford, England)
|August 31, 2001
Summary
Cryoloop vitrification effectively preserves Rhesus monkey blastocysts. Procedure B, using glycerol and ethylene glycol, yielded high survival and hatching rates, outperforming conventional cooling and demonstrating successful pregnancy outcomes.
Area of Science:
- Reproductive Biology
- Cryobiology
- Primate Embryology
Background:
- Evaluating vitrification for non-human primate blastocyst preservation.
- Comparing cryoloop procedure with controlled rate cooling.
Purpose of the Study:
- Assess two distinct cryoprotectant mixtures for vitrification.
- Determine optimal conditions for Rhesus monkey blastocyst cryopreservation.
Main Methods:
- Rhesus monkey blastocysts produced via intracytoplasmic sperm injection.
- Vitrification using cryoloop with two different cryoprotectant solutions (A and B).
- Cryoprotectant removal via stepwise sucrose solutions and co-culture on buffalo rat liver cells.
Main Results:
- Procedure B (glycerol/ethylene glycol) resulted in 85% survival and 71% hatching.
- Procedure A showed 38% survival and minimal hatching.
- Conventional slow cooling yielded 36% survival and 6% hatching.
- Successful twin-term pregnancy achieved after embryo transfer from Procedure B.
Conclusions:
- Modified cryoloop vitrification with glycerol and ethylene glycol is effective for Rhesus monkey blastocysts.
- This method is simple, rapid, and cost-effective for blastocyst preservation.