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Reverse genetics of influenza virus
1Department of Pathobiological Sciences, School of Veterinary Medicine, Madison, Wisconsin 53706, USA.
Virology
|September 5, 2001
Summary
Reverse genetics allows generating viruses from cloned DNA. New methods now enable influenza virus reverse genetics, advancing virus research.
Area of Science:
- Virology
- Molecular Biology
Background:
- Reverse genetics has advanced for negative-sense RNA viruses, enabling virus generation from cloned complementary DNA (cDNA).
- Generating segmented genome viruses, like influenza, lagged due to technical challenges in providing all viral RNAs and proteins from cDNA.
- A significant breakthrough occurred in 1999 with new approaches to viral RNA production.
Purpose of the Study:
- To review the background and advancements in reverse genetics for influenza virus.
- To discuss available systems for generating influenza viruses using reverse genetics.
- To explore the implications of these developments for future virus research.
Main Methods:
- Review of literature on reverse genetics techniques for negative-sense RNA viruses.
- Focus on the 1999 breakthrough enabling influenza virus reverse genetics.
- Discussion of current systems for influenza virus generation.
Main Results:
- Established methods for reverse genetics of nonsegmented negative-sense RNA viruses.
- Development of novel systems for influenza virus reverse genetics since 1999.
- Facilitation of virus generation from cloned cDNA for segmented RNA viruses.
Conclusions:
- Reverse genetics systems for influenza virus have significantly progressed.
- These advancements provide powerful tools for influenza virus research.
- Future research will benefit from the ability to generate and manipulate influenza viruses more readily.