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Pulmonary lipid phosphate phosphohydrolase in plasma membrane signalling platforms
1Department of Biochemistry, Health Sciences Building, The University of Western Ontario, London, ON, Canada N6A 5C1.
The Biochemical Journal
|September 6, 2001
Summary
Lipid phosphate phosphohydrolase (LPP) is found in lung cell lipid rafts, suggesting a role in cell signaling and caveolar dynamics. This localization is consistent with LPP
Area of Science:
- Cell Biology
- Biochemistry
- Signal Transduction
Background:
- Lipid phosphate phosphohydrolase (LPP) is implicated in signal transduction and cellular proliferation.
- LPP may act sequentially with phospholipase D (PLD).
Purpose of the Study:
- To investigate the localization and function of LPP in lung tissue and cell lines.
- To determine if LPP is associated with lipid-rich signaling platforms like caveolae and rafts.
Main Methods:
- Isolation of caveolin-enriched domains (CEDs) from rat lung tissue and epithelial cell lines (MLE12, MLE15).
- Biochemical assays to measure LPP activity.
- Biochemical analysis of lipid and protein content in isolated domains.
- Cellular treatments including saponin and phorbol ester.
- Analysis of LPP and caveolin-1 during type II cell transdifferentiation.
Main Results:
- LPP activity is enriched in lipid-rich signaling platforms (caveolae/rafts) from lung tissue and type II cells.
- LPP3 isoform is predominantly found in rat lung CEDs.
- Saponin treatment disrupts these microdomains, altering LPP localization.
- Activated LPP1/1a protein may be recruited to caveolae/rafts.
- Transdifferentiation of type II cells shows increased LPP activity and caveolin-1.
Conclusions:
- LPP is localized in caveolae and/or rafts within lung tissue and related cell lines.
- This localization supports a role for LPP in caveolae/raft-mediated signaling.
- LPP may also be involved in the dynamics of caveolae.