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Related Experiment Videos

Controlling denitrification in closed artificial ecosystems.

R L Mancinelli1, D T Smernoff, M R White

  • 1NASA-Ames Research Center, Moffett Field, CA 94035, USA.

Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)
|September 7, 2001
PubMed
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Denitrification, the microbial conversion of nitrate to nitrogen gas, occurs even in oxygen-rich closed systems. This study shows Pseudomonas fluorescens denitrifies under aerobic conditions, impacting closed system efficiency.

Area of Science:

  • Microbiology
  • Environmental Science
  • Biotechnology

Background:

  • Denitrification is the microbial reduction of nitrate to gaseous nitrogen forms (N2O, N2).
  • Closed artificial systems, like plant growth chambers, experience nitrogen loss via denitrification.
  • Denitrification reduces system efficiency and releases nitrous oxide (N2O), a greenhouse gas.

Purpose of the Study:

  • To investigate the effect of oxygen levels on denitrification in closed systems.
  • To study the population growth of Pseudomonas fluorescens under varying oxygen conditions.
  • To assess the potential of Pseudomonas fluorescens to mitigate denitrification in closed environments.

Main Methods:

  • Utilized Pseudomonas fluorescens (ATCC # 17400) as a model organism.
Keywords:
NASA Center ARCNASA Discipline Life Support Systems

Related Experiment Videos

  • Grew the bacteria in a two-liter bioreactor under controlled, varying oxygen levels.
  • Monitored population growth and denitrification rates in response to oxygen availability.

    • Main Results:

    • Denitrification was observed in the closed system even under conditions considered aerobic.
    • Pseudomonas fluorescens exhibited denitrification despite sufficient oxygen supply and rapid gas turnover.
    • Oxygen levels significantly influence bacterial growth and denitrification activity in closed systems.

    Conclusions:

    • Denitrification can occur in well-mixed, oxygen-rich closed systems, contrary to common assumptions.
    • Pseudomonas fluorescens actively denitrifies under aerobic conditions, posing challenges for closed system management.
    • Understanding oxygen's role is crucial for controlling denitrification and optimizing closed artificial ecosystems.