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W-reactivation and W-mutagenesis in phage phi X 174
F Yatagai1, T Takahashi, S Kitayama
1The Institute of Physical and Chemical Research, Wako-shi, Japan.
Abstract:
W-reactivation (WR) and W-mutagenesis (WM) were observed after irradiation of phage chi X 174 with He and N ions (4.6 MeV/amu). The relative amount of phage RFI DNA (RARFI) extracted from the infected cells was determined by agarose gel electrophoresis. It was found that RARFI was increased by prior UV-irradiation of host cells and this increase corresponded to the efficiency of WR.