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A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
12:11

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Published on: July 9, 2012

Sensitive detection of Cryptosporidium oocysts in environmental water samples by reverse transcription-PCR

T Karasudani1, S Aoki, J Takeuchi

  • 1Ehime Prefectural Institute of Public Health and Environmental Science, Sanban-cho 8-234, Matsuyama, Ehime 790-0003, Japan. karasudani-tatsuya@pref.ehime.jp

Japanese Journal of Infectious Diseases
|September 7, 2001
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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