Related Experiment Video
Updated: Aug 18, 2026

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
Published on: June 25, 2010
Determination of carbohydrates by capillary electrophoresis with electrospray-mass spectrometric detection
1Department of Analytical Chemistry, Johannes Kepler-University Linz, Austria. christian.klampfl@jk.uni-linz.ac.at
Abstract:
A method for the determination of underivatized carbohydrates using capillary electrophoresis (CE) with detection by electrospray ionization-mass spectrometry (ESI-MS) presented. Highly alkaline carrier electrolytes based on volatile organic bases like is diethylamine (DEA) combined with MS detection in the negativ-ion mode proved to be the optimum solution for the separation and detection of these analytes. Optimization of the carrier electrolyte composition has been performed with respect to its pH, ionic strength as well as the addition of an organic modifier. The influence of the DEA concentration in the sheath liquid on parameters like peak shapes or signal-to-noise (S/N) ratios was also investigated. Limits of detection (LOD) were in the range of 0.5-3.0 mgL(-1) and calibration was linear over an order of magnitude for almost all solutes investigated. Finally, the applicability of this method for the analysis of real samples was demonstrated with wine samples.
More Related Videos
07:46Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
06:13Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method
Published on: March 31, 2022
Related Concept Videos
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
High-Resolution Mass Spectrometry (HRMS)
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Electrophoresis: Overview
There...
Capillary Electrophoresis: Instrumentation
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...