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Combined treatment with vitamin B12b and ascorbic acid causes in vitro DNA degradation in tumor cells
A I Medvedev1, V S Akatov, N D Kreshchenko
1Laboratory of Cell Biochemistry and Bioenergetics, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino.
Abstract:
Incubation of Ehrlich ascites carcinoma and HEp-2 human epidermoid laryngeal carcinoma cells with hydroxycobalamin (vitamin B12b) and ascorbic acid induced generation and accumulation of double-stranded DNA fragments (23,000 b.p. and longer) in cells. The same vitamins alone in the same concentrations produced no such effects. DNA degradation in HEp-2 cells caused by long-term (4 h) incubation with 5-25 microM hydroxycobalamin and ascorbic acid (1:10-1:40 molar ratio) at 37 degrees C was comparable with that induced by gamma-irradiation in a dose of 150 Gy at 4 degrees C.