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Dissecting and Recording from The C. Elegans Neuromuscular Junction
Published on: February 25, 2009
Effect of cholinergic agonists on resting membrane potential of earthworm body wall muscle cells
E M Volkov1, L F Nurullin, V N Frosin
1Kazan State Medical University. emvolkov@kzn.ru
Abstract:
Carbachol in a concentration of 5x10(-8) mol/liter does not hyperpolarize, and in a concentration of 5x10(-6) mol/liter depolarizes the membrane of somatic muscle cell in earthworm. d-tubocurarine, alpha-bungarotoxin, atropine, and hexamethonium added to the incubation medium did not abolish the carbachol-induced decrease in resting membrane potential. Each of these drugs alone had no effect on resting membrane potential in muscle cells. Presumably, the acetylcholine-sensitive receptor-channel membrane complex in earthworm muscle cell differs from acetylcholine receptor in skeletal muscle fibers and peripheral neurons of vertebrates.
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