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Updated: Oct 4, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Free radical lipid peroxidation inhibits enzymatic conversion of beta-carotene into vitamin A
N N Gessler1, S B Gomboeva, K B Shumaev
1Laboratory of Biochemistry and Biotechnology of Low-Molecular-Weight Natural Compounds, A. N. Bakh Institute of Biochemistry, Russian Academy of Sciences.
Abstract:
Free radical oxidation of arachidonic acid with soybean lipoxygenase was accompanied by inhibition of retinal synthesis from beta-carotene catalyzed by enzyme preparation from rabbit intestinal mucosa. Lipoxygenase inhibitor salicylhydroxamic acid and antioxidants suppressing free radical reactions (ethyl gallate, alpha-tocopherol, astaxanthine, and quercetin) promoted conversion of beta-carotene into retinal catalyzed by beta-carotene-15,15'-dioxygenase. These results indicate that lipoperoxides and/or products of their homolysis attenuate enzymatic conversion of beta-carotene and confirm the important role of natural antioxidants in the maintenance of stable vitamin A content in mammals.
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